Uncovering Gender and Age Neutrality in CT Values for E and RdRp Gene Amplification in SARS-CoV-2

Ibrahim A. Naqid; Masood Ahmed Hameed; Nawfal R. Hussein; Shivan Hassan Yousif

doi:10.59786/bmtj.125

Authors

Ibrahim A. Naqid Department of Biomedical Sciences, College of Medicine, University of Zakho, Zakho, 42002, Iraq https://orcid.org/0000-0002-7223-2690
Masood Ahmed Hameed Department of Biomedical Sciences, College of Medicine, University of Zakho, Zakho, 42002, Iraq https://orcid.org/0009-0004-3883-3590
Nawfal R. Hussein Department of Biomedical Sciences, College of Medicine, University of Zakho, Zakho, 42002, Iraq https://orcid.org/0000-0002-7813-9198
Shivan Hassan Yousif Department of Biomedical Sciences, College of Medicine, University of Zakho, Zakho, 42002, Iraq https://orcid.org/0009-0001-3916-1640

DOI:

https://doi.org/10.59786/bmtj.125

Keywords:

COVID-19, SARS-CoV-2, RdRp gene, E gene, RT-PCR

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic poses a danger to public health across the world. Specific and sensitive testing is vital to tracking and containing the infected individuals. This study examines the characteristics of 705 patients based on gender and age groups, with a focus on their CT (Cycle Threshold) values associated with E- and RdRp gene amplification. Among the patient cohort, 58.87% were male, while 41.13% were female. Notably, all males and females tested positive for the E gene, achieving a 100% detection rate, whereas the RdRp gene exhibited a slightly lower positive rate of 93.4% in males and 89.6% in females. Statistical analysis through the chi-square test revealed no significant disparities between target genes and gender. Additionally, a one-way ANOVA test demonstrated no significant distinctions between target genes and age groups. This study's findings suggest that gender and age do not significantly influence CT values for E and RdRp gene amplification, and there is no preferred gene for detection, providing valuable insights into the molecular characteristics of the studied cohort.

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